EXPLORATION AND PROFILING OF PROBABLE HALOPHILES: CHARACTERIZATION AND IDENTIFICATION
Abstract
This study focuses on the exploration and profiling of probable halophiles, microorganisms that thrive in high-salinity environments. The research aims to characterize and identify these extremophiles through a series of laboratory-based analyses. Samples were collected from various saline environments, including salt pans and hypersaline lakes. Initial screening involved culturing techniques to isolate halophilic strains, which were then subjected to morphological and biochemical assessments. Advanced characterization methods, including molecular techniques such as 16S rRNA gene sequencing and physiological testing, were employed to accurately identify these microorganisms and elucidate their salinity tolerance mechanisms. Results revealed a diverse array of halophilic organisms, with significant variations in salt tolerance and biochemical profiles. This study provides valuable insights into the diversity of halophiles and their adaptive strategies, contributing to a better understanding of microbial life in extreme environments. The findings also have potential applications in biotechnology and environmental management, where halophiles may offer novel solutions for saline waste treatment and other industrial processes.
Keywords
halophiles, extremophiles, saline environments, microbial characterizationHow to Cite
Downloads
References
Ara I, Daram D, Baljinova T, Yamamura H, Bakir MA, Suto M, et al. Isolation classification phylogenetic analysis and scanning electron microscopy of halophilic halotolerant and alkaliphilic actinomycetes isolated from hypersaline soil. African Journal of Microbiology Research. 2013; 7(4):298-308.
DasSarma S, Arora P. Encyclopaedia of life science. Nature Publishing Group; London. Halophiles, 2001, 1-9.
Gilmour D. Halotolerant and halophilic microorganisms. In: microbiology of extreme environments. Edwards C.(Ed.). McGraw – Hill NY USA, 1990, 147-177.
Gonzalez-Toril E, Llobet- Brossa E, Casamayor EO, Amann R, Amils R. Microbial ecology of an extreme acidic environment the Tinto River. Applied and Environmental Microbiology. 2003; 69(8):4853-4865.
Holm GE, Sheman JM. Salt effects in bacterial growth IV. The physical nature of bacterial growth in various concentrations of neutral salts. Experimental Biology and Medicine. 1924; 21(6):31-315.
Jensen LB. Introduction and History. In: Microbiology of meats 3rd edition. The Garrard press Champaign. Illinois, 1954, 1-11.
Johnson AM, Thurlow LM, Zwenger SR, Gillock ET.Partial Characterization of Two Moderately Halophilic Bacteria from a Kansas Salt Marsh. The Prairie Naturalist. 2007; 39 (1):29-39.
Koo I. Using Salt as a Food Preservative; Misconceptions about Preserving Foods with Salt, 2009.
Kushner DJ. Growth and nutrition of halophilic bacteria. In: The biology of halophilic bacteria. (R. H. Vreeland and L. I. Hochstein (eds).CRC Press. Boca Raton Florida, 2002, 87-104
Oren A. Diversity of halophilic microorganisms: Environments phylogeny physiology and applications. Journal of Industrial Microbiology & Biotechnology.2002; 28:56-63.
Parish M. How do salt and sugar prevent microbial spoilage? Sci. Am. 2006; 294(5):98
Seckbach J, Oren A, Chela-Flores J. The extreme environments and their microbes as models for extraterrestrial life. 3rd European Planetary Science Congress Münster Germany September, 2008, 853
Copyright (c) 2024 JOSEPH UBOSI, JENNIFER UDEGBE, WILLIAMS OKOUGH, MARGARET ALANA (Author)
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors retain the copyright of their manuscripts, and all Open Access articles are disseminated under the terms of the Creative Commons Attribution License 4.0 (CC-BY), which licenses unrestricted use, distribution, and reproduction in any medium, provided that the original work is appropriately cited. The use of general descriptive names, trade names, trademarks, and so forth in this publication, even if not specifically identified, does not imply that these names are not protected by the relevant laws and regulations.
